0.5M EDTA

Na2EDTA- dihydrate 186.1 g
Milli-Q Water ~1 L
NaOH ~20 g
  • Add EDTA into 800 mL Milli-Q water (NOTE: EDTA will not dissolve)
  • Using NaOH pellets, adjust pH to 8.0 (EDTA should now dissolve)
  • Bring volume to 1 L with Milli-Q
  • Autoclave for 30 minutes
  • Store at 4oC