| Na2EDTA- dihydrate | 186.1 g |
| Milli-Q Water | ~1 L |
| NaOH | ~20 g |
- Add EDTA into 800 mL Milli-Q water (NOTE: EDTA will not dissolve)
- Using NaOH pellets, adjust pH to 8.0 (EDTA should now dissolve)
- Bring volume to 1 L with Milli-Q
- Autoclave for 30 minutes
- Store at 4oC