1M Tris

Tris Base 121.1 g
Milli-Q Water ~1 L
HCl ~42 mL
  •  Dissolve Tris base into 800 mL Milli-Q water
  • Using HCl, adjust pH to 8.0
  • Bring volume to 1 L using Milli-Q
  • Autoclave for 30 minutes (NOTE: re-make if solution turns yellow)
  • Store at 4oC