| Tris Base | 121.1 g |
| Milli-Q Water | ~1 L |
| HCl | ~42 mL |
- Dissolve Tris base into 800 mL Milli-Q water
- Using HCl, adjust pH to 8.0
- Bring volume to 1 L using Milli-Q
- Autoclave for 30 minutes (NOTE: re-make if solution turns yellow)
- Store at 4oC
| Tris Base | 121.1 g |
| Milli-Q Water | ~1 L |
| HCl | ~42 mL |