25% Ringer Solution

NaCl 2.25 g
KCl 105 mg
CaCl2 45 mg
NaHCO3 50 mg
Citric Acid 34 mg
Milli-Q Water ~1 L
  • Measure out approximately 300 mL Milli-Q
  • Add all components in order
  • Bring volume up to 1 L with Milli-Q
  • Autoclave for 45 minutes