-Pi stock | 25 mL |
+Pi stock | 6.25 mL |
Agar | 10 g |
Streptomycin Stock (1 mg/mL) | 1 mL |
Neomycin Stock (25 mg/mL) | 1 mL |
Milli-Q Water | ~1 L |
- Measure out approximately 300 mL Milli-Q
- Shake -Pi stock
- Add both stocks to water
- Bring volume up to 1 L with Milli-Q
- Check pH. If less than 7.8, make fresh +Pi
- Add agar
- Autoclave for 20 minutes
- Add antibiotics
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