Allen and Arnon Medium (Plates)

-Pi stock 25 mL
+Pi stock 6.25 mL
Agar 10 g
Streptomycin Stock (1 mg/mL) 1 mL
Neomycin Stock (25 mg/mL) 1 mL
Milli-Q Water ~1 L
  • Measure out approximately 300 mL Milli-Q
  • Shake -Pi stock
  • Add both stocks to water
  • Bring volume up to 1 L with Milli-Q
  • Check pH. If less than 7.8, make fresh +Pi
  • Add agar
  • Autoclave for 20 minutes
  • Add antibiotics