| -Pi stock | 25 mL |
| +Pi stock | 6.25 mL |
| Agar | 10 g |
| Streptomycin Stock (1 mg/mL) | 1 mL |
| Neomycin Stock (25 mg/mL) | 1 mL |
| Milli-Q Water | ~1 L |
- Measure out approximately 300 mL Milli-Q
- Shake -Pi stock
- Add both stocks to water
- Bring volume up to 1 L with Milli-Q
- Check pH. If less than 7.8, make fresh +Pi
- Add agar
- Autoclave for 20 minutes
- Add antibiotics