| NaCl | 25 g |
| MgCl2•6H2O | 2 g |
| KCl | 500 mg |
| NaNO3 | 750 mg |
| K2HPO4•3H2O | 20 mg |
| MgSO4•7H2O | 3.5 g |
| CaCl2•2H2O | 500 mg |
| Citric Acid | 3 mg |
| Ferric Ammonium Citrate | 3 mg |
| EDTA (disodium magnesium) | 0.5 mg |
| Na2CO3 | 20 mg |
| Trace Metal Solution | 1 mL |
| Milli-Q Water | ~1 L |
- Measure out approximately 300 mL Milli-Q
- Add all components in order
- Bring volume up to 1 L with Milli-Q
- Autoclave for 20 minutes
pH: 7.5