ASNIII

NaCl 25 g
MgCl2•6H2O 2 g
KCl 500 mg
NaNO3 750 mg
K2HPO4•3H2O 20 mg
MgSO4•7H2O 3.5 g
CaCl2•2H2O 500 mg
Citric Acid 3 mg
Ferric Ammonium Citrate 3 mg
EDTA (disodium magnesium) 0.5 mg
Na2CO3 20 mg
Trace Metal Solution 1 mL
Milli-Q Water ~1 L
  • Measure out approximately 300 mL Milli-Q
  • Add all components in order
  • Bring volume up to 1 L with Milli-Q
  • Autoclave for 20 minutes

pH: 7.5