| Ammonium Sulfate | 1.32 mg |
| MgSO4•7H2O | 380 mg |
| CaCl2•2H2O | 20 mg |
| MnCl2•4H2O, 50.5 mM solution | 20 µL |
| Na2MoO4•2H2O, 41.3 mM solution | 10 µL |
| CoCl2•6H2O, 1.7 mM solution | 5 µL |
| ZnSO4•7H2O, 34.8 mM solution | 10 µL |
| K2HPO4 | 87 mg |
| Phenol red 0.5% | 250 µL |
| Iron Solution | 250 µL |
| Milli-Q Water | ~1 L |
- Measure out approximately 300 mL Milli-Q
- Add all components in order
- Bring volume up to 1 L with Milli-Q
- Adjust pH to 7.5 with 0.5M K2CO3
- Autoclave for 20 minutes
pH: 7.5