ATCC Culture Medium 929

Ammonium Sulfate 1.32 mg
MgSO4•7H2O 380 mg
CaCl2•2H2O 20 mg
MnCl2•4H2O, 50.5 mM solution 20 µL
Na2MoO4•2H2O, 41.3 mM solution 10 µL
CoCl2•6H2O, 1.7 mM solution 5 µL
ZnSO4•7H2O, 34.8 mM solution 10 µL
K2HPO4 87 mg
Phenol red 0.5% 250 µL
Iron Solution 250 µL
Milli-Q Water ~1 L
  • Measure out approximately 300 mL Milli-Q
  • Add all components in order
  • Bring volume up to 1 L with Milli-Q
  • Adjust pH to 7.5 with 0.5M K2CO3
  • Autoclave for 20 minutes

pH: 7.5