NaNO3 1.5 g
K2HPO4•3H2O 40 mg
MgSO4•7H2O 75 mg
CaCl2•2H2O 36 mg
Citric Acid 6 mg
Ferric Ammonium Citrate 6 mg
EDTA (disodium magnesium) 1 mg
Na2CO3 20 mg
Trace Metal Solution 1 mL
Milli-Q Water ~1 L
  • Measure out approximately 300 mL Milli-Q
  • Add all components in order
  • Bring volume up to 1 L with Milli-Q
  • Autoclave for 20 minutes

(note: Do Not Autoclave if making concentrated stock solutions!)

pH: 7.4

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