| Na2-EDTA | 1 mg |
| Citric Acid | 6 mg |
| K2HPO4•3H2O | 40 mg |
| MgSO4•7H2O | 75 mg |
| CaCl2•2H2O | 36 mg |
| Na2CO3 (H2O) | 20 mg |
| Ferric Ammonium Citrate | 6 mg |
| Trace Metal Solution | 50 µL |
| Milli-Q Water | ~1 L |
- Measure out approximately 300 mL Milli-Q
- Add all components in order
- Bring volume up to 1 L with Milli-Q
- Autoclave for 20 minutes
(note: Do Not Autoclave if making concentrated stock solutions!)