Cutting the Bands
- Fill appropriate number of 500 µL PCR tubes with 50 µL of 10 mM Tris buffer
- Carefully slide the gel onto the wetted transilluminator plate (wetting the edges of the gel is helpful)
- Wearing protective equipment, turn on the transmitted UV switch
- Using a sterilized scalpel (cleaned with 70% ethanol), cut along the top and bottom edges of the band
- Snip the left and right edges of the band with sterilized forceps (cleaned with 70% ethanol)
- Remove the excised band and place in corresponding PCR tube
- Repeat as necessary
- Store tubes at 4oC for three to five days to allow DNA to diffuse into the buffer
Pingback: Leandro Farland
Pingback: Reba Fleurantin
Pingback: Chirurgie Tunisie
Pingback: Chirurgiens esthétique Tunisie
Pingback: Chirurgiens esthétique Tunisie
Pingback: National Chi Nan University
Pingback: Scientific research and publishing
Pingback: Research opportunities
Pingback: تنسيق الجامعات الخاصة
Pingback: Alumni network
Pingback: Bachelor degrees in HRM
Pingback: MBA in FUE
Pingback: Intellectual independence
Pingback: Political science
Pingback: Social Media Program
Pingback: طرق التعلم في الصيدلة
Pingback: Pharmacology and Toxicology
Pingback: منتجات الرعاية الصحية
Pingback: Dentistry education
Pingback: Academic Year
Pingback: academic programs at the College of Engineering
Pingback: الانسحاب من الفصل الدراسي
Pingback: Online Learning
Pingback: top university egypt
Pingback: future unversity in egypt news
Pingback: regional centers for software
Pingback: Biochemistry
Pingback: دورات التعليم المستمر لطب الأسنان
Pingback: Faculty of dentistry
Pingback: برامج الدراسات العليا في جامعة المستقبل
Pingback: تحويل قبول الطلاب إلى جامعة المستقبل
Pingback: Letters of recommendation for future university
Pingback: خطابات توصية لجامعة المستقبل
Pingback: fue
Pingback: البحث العلمي
Pingback: قسم الكيمياء الصيدلانية
Pingback: ماجستير جراحة الوجه والفكين كم سنه
Pingback: Pharmaceutical Analytical Chemistry
Pingback: Information Technology Education
Pingback: Academic Appeals