Stock
| NTA | 2 g |
| NaNO3 | 14 g |
| KNO3 | 2 g |
| Na2HPO4 | 2.2 g |
| MgSO4•7H2O | 2 g |
| CaSO4•2H2O | 1.2 g |
| NaCl | 160 mg |
| Iron Solution | 20 mg |
| Micronutrient Solution | 10 mL |
| Milli-Q Water | ~1 L |
- Measure out ~500 mL Milli-Q Water
- Add components in order
- Bring volume to 1 L with Milli-Q
- Adjust pH to 8.2
- Autoclave for 20 minutes
Media
| D Medium Stock | 50 mL |
| Milli-Q Water | ~1 L |
- Measure out ~500 mL Milli-Q
- Add D Medium Stock
- Bring volume to 1 L with Milli-Q
- Adjust pH to 8.2
- Either add 1% agar, autoclave for 20 minutes and pour plates, or aliquote into erlenmeyer flasks and autoclave for 20 minutes
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