D Medium

Stock

NTA 2 g
NaNO3 14 g
KNO3 2 g
Na2HPO4 2.2 g
MgSO4•7H2O 2 g
CaSO4•2H2O 1.2 g
NaCl 160 mg
Iron Solution 20 mg
Micronutrient Solution 10 mL
Milli-Q Water ~1 L
  • Measure out ~500 mL Milli-Q Water
  • Add components in order
  • Bring volume to 1 L with Milli-Q
  • Adjust pH to 8.2
  • Autoclave for 20 minutes

Media

D Medium Stock 50 mL
Milli-Q Water ~1 L
  •  Measure out ~500 mL Milli-Q
  • Add D Medium Stock
  • Bring volume to 1 L with Milli-Q
  • Adjust pH to 8.2
  • Either add 1% agar, autoclave for 20 minutes and pour plates, or aliquote into erlenmeyer flasks and autoclave for 20 minutes