D Medium

Stock

NTA 2 g
NaNO3 14 g
KNO3 2 g
Na2HPO4 2.2 g
MgSO4•7H2O 2 g
CaSO4•2H2O 1.2 g
NaCl 160 mg
Iron Solution 20 mg
Micronutrient Solution 10 mL
Milli-Q Water ~1 L
  • Measure out ~500 mL Milli-Q Water
  • Add components in order
  • Bring volume to 1 L with Milli-Q
  • Adjust pH to 8.2
  • Autoclave for 20 minutes

Media

D Medium Stock 50 mL
Milli-Q Water ~1 L
  •  Measure out ~500 mL Milli-Q
  • Add D Medium Stock
  • Bring volume to 1 L with Milli-Q
  • Adjust pH to 8.2
  • Either add 1% agar, autoclave for 20 minutes and pour plates, or aliquote into erlenmeyer flasks and autoclave for 20 minutes

44 thoughts on “D Medium”

  1. Pingback: Arie Baisch

  2. Pingback: Lincoln Georgis

  3. Pingback: Chirurgie Tunisie

  4. Pingback: Chirurgie esthétique Tunisie

  5. Pingback: Chirurgie esthétique Tunisie

  6. Pingback: National Chi Nan University

  7. Pingback: Academic programs

  8. Pingback: Scholarships to Study in Egypt

  9. Pingback: صفحة جامعة المستقبل

  10. Pingback: FUE Chairman

  11. Pingback: Inspiring academic environment

  12. Pingback: Higher education in Human Resources Management

  13. Pingback: Finance courses

  14. Pingback: Finance research

  15. Pingback: fue

  16. Pingback: Problem-solving skills

  17. Pingback: علوم السياسة

  18. Pingback: Hardness Tester

  19. Pingback: learning methods in pharmacy

  20. Pingback: fue

  21. Pingback: Research Activities

  22. Pingback: Pharmaceutics and Pharmaceutical Technology

  23. Pingback: Clinical dentistry

  24. Pingback: Academic Advisor

  25. Pingback: Master's in prosthodontics

  26. Pingback: علم الاسنان

  27. Pingback: الحياة الطلابية بكلية الهندسة

  28. Pingback: البحث والابتكار

  29. Pingback: Bachelor's Degree in Software Engineering

  30. Pingback: Computer Science

  31. Pingback: best university in egypt

  32. Pingback: Higher education

  33. Pingback: scientific approach

  34. Pingback: comprehensive reform

  35. Pingback: Executive MBA program in Cairo

  36. Pingback: برامج البكالوريوس في جامعة المستقبل

  37. Pingback: ما هي افضل الكليات الخاصه

  38. Pingback: درجات البكالوريوس في الموارد البشرية

  39. Pingback: fue

  40. Pingback: كلية طب الأسنان

  41. Pingback: Graduation project

  42. Pingback: Attendance Policy

  43. Pingback: The University of Cork

  44. Pingback: العقاقير

Comments are closed.