DGGE Standards

Materials

ND1-1
ATTC 29133
S1006 (diatom)
MoBio Plant DNA Extraction Kit

or

Materials for PCI Method

Procedure

Extract DNA of all samples using either the MoBio Plant Kit or PCI Extraction method

Amplify DNA with cyanobacterial GC-clamped primers using the standard 30 cycle amplification for cyanobacteria

NOTE: These strains have all been shown to give only one band

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