IMR Saltwater Medium

NaNO3 (4.25 g/L) 10 mL
NaH2PO4 (690 mg/L) 7 mL
Trace Metal Stock 1 mL
Vitamin Stock 500 µL
Sea Water (filtered) 1 L
  • Measure out sea water
  • Add first three components in order
  • Bring volume up to 1 L with Milli-Q
    • (Optional: Add 10 g Gelrite for solid medium)
  • Autoclave for 20 minutes
  • Add vitamin stock
  • Store at 4oC