| NaNO3 (4.25 g/L) | 10 mL |
| NaH2PO4 (690 mg/L) | 7 mL |
| Trace Metal Stock | 1 mL |
| Vitamin Stock | 500 µL |
| Sea Water (filtered) | 1 L |
- Measure out sea water
- Add first three components in order
- Bring volume up to 1 L with Milli-Q
- (Optional: Add 10 g Gelrite for solid medium)
- Autoclave for 20 minutes
- Add vitamin stock
- Store at 4oC