| Isoamyl Alcohol | 5 mL |
| Chloroform | 120 mL |
| Phenol | 125 mL |
- Put on gloves, cover forearms, and relocate to fume hood
- Combine all components in sterile 250 mL glass bottle
- Mix well
- Wrap container in foil to protect from light
- Store at 4oC
| Isoamyl Alcohol | 5 mL |
| Chloroform | 120 mL |
| Phenol | 125 mL |