Cyanobacterial Primers (cyano)
First Cycle (run once)
- 95oC for 5 minutes
- 80oC for 1 minute
- With about 3 seconds remaining, pause thermocycler and add Taq
Second Cycle (run 30 times)
- 94oC for 1 minute
- 60oC for 1 minute
- 72oC for 1 minute
Third Cycle (run once)
- 72oC for 9 minutes
- 4oC hold
Eubacterial Primers (eubac td)
First Cycle (run once)
- 95oC for 5 minutes
- 80oC for 1 minute
- With about 3 seconds remaining, pause thermocycler and add Taq
- 65oC for 1 minute
- 75oC for 3 minutes
Second Cycle, Touchdown PCR (run 20 times)
- 94oC for 1 minute
- 65oC for 1 minute
- 75oC for 3 minute
- NOTE: this is a touchdown cycle; the temperature for the second step must be lowered by 1oC every second cycle (ending on 55oC).
Third Cycle (run 9 times)
- 94oC for 1 minute
- 55oC for 1 minute
- 72oC for 3 minute
Fourth Cycle (run once)
- 72oC for 7 minutes
- 4oC hold
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