• PCR tubes (one per sample, two for controls, and one extra)
  • Two 2 mL microcentrifuge tubes
  • Template
  • E. coli DNA (or other positive control)
  • Primers, forward and reverse
  • 10% BSA (LF)
  • 10x Ex Taq buffer
  • dNTP mixture
  • 5x Eppendorf Enhancer
  • Milli-Q water, autoclaved
  • Ex Taq DNA polymerase
  • Assorted pipettors and tips


  • Open all PCR and microcentrifuge tubes and place in racks found in the PCR hood, along with all pipettors and tips (note: all pipettors should already be in hood)
  • Close the side panels and set the UV light timer for 20 minutes
  • Turn on UV light and blower for the flow hood
  • Gather all reagents and samples, placing them in an ice bath
  • Enhancer should be placed in a warm water bath to dissolve precipitate
  • Enter relevant data into the “Master Mix” calculation spreadsheet; make a print out
  • In PCR hood, prepare master mix in one 2 mL microcentrifuge tube
  • Aliquot master mix into PCR tubes
  • Transfer PCR tubes to ice bath and move to flow hood
  • Add template to each reaction tube, E. coli (or equivalent) to positive control, and PCR water to negative control
  • Place caped and labeled tubes in PCR thermocycler, then close lid
  • Turn on thermocycler, select desired PCR cycle, and begin
  • Pause thermocycler at very end of second cycle
  • Quickly add Taq polymerase to each tube (note: Do not expel polymerase from tip; it will flow out automatically. Do not allow any air bubbles to form in PCR tube.)
  • Close thermocycler lid and press “resume”
  • Store tubes at 4oC

47 thoughts on “PCR

  1. Pingback: Arie Baisch

  2. Pingback: Lincoln Georgis

  3. Pingback: Chirurgiens esthétique Tunisie

  4. Pingback: Chirurgie Tunisie

  5. Pingback: Chirurgie esthétique Tunisie

  6. Pingback: National Chi Nan University

  7. Pingback: التطوير المستمر

  8. Pingback: Academic freedom

  9. Pingback: Higher education

  10. Pingback: افضل كلية سياسة في مصر

  11. Pingback: Attitudes and behavior

  12. Pingback: Water Bath

  13. Pingback: الكيمياء التحليلية الصيدلانية

  14. Pingback: Welcome Note for faculty of pharmacy

  15. Pingback: Large Lecture Halls

  16. Pingback: Social Activities for pharmacy students at future university

  17. Pingback: طب أسنان الأطفال

  18. Pingback: لائحة طب الفم والاسنان

  19. Pingback: Get in Touch with Faculty of Oral and Dental Medicine

  20. Pingback: Admissions requirements for dentistry master's programs

  21. Pingback: نظام الامتحانات

  22. Pingback: teaching and student activities

  23. Pingback: وظائف هندسية

  24. Pingback: الهندسة الكهربائية

  25. Pingback: Information Technology Education

  26. Pingback: برامج تكنولوجيا المعلومات في مصر

  27. Pingback: industry partnerships

  28. Pingback: كلية الحاسبات والمعلومات

  29. Pingback: Webmaster

  30. Pingback: QS World University Rankings

  31. Pingback: top university in egypt

  32. Pingback: awarding joint degrees

  33. Pingback: Prof. Hesham Arafat

  34. Pingback: Future University Egypt MBA

  35. Pingback: ماجستير في إدارة الأعمال في FUE

  36. Pingback: علم الأحياء الدقيقة والمناعة

  37. Pingback: Transcript requirements for future university

  38. Pingback: Transfer students admissions to future university

  39. Pingback: Application deadlines for future university

  40. Pingback: Higher education in Marketing

  41. Pingback: الأخلاقيات المهنية

  42. Pingback: تطوير الحياة المهنية

  43. Pingback: التدويل

  44. Pingback: Dentistry education

  45. Pingback: Sustainable Development

  46. Pingback: best university in egypt

  47. Pingback: Dental Residency Programs

Comments are closed.