| NaNO3 | 50 mg |
| Na2-Glycerolphosphate•4H2O | 50 mg |
| Tris Buffer | 500 mg |
| Iron Solution | 25 mL |
| Trace Metal | 15 mL |
| Milli-Q Water | 100 mL |
- Measure out Milli-Q
- Add NaNO3 and Na2-Glycerolphosphate•4H2O
- Combine trace metal and iron solutions in separate container
- Autoclave both containers
- Once cool, combine both mixtures
- Store at 4oC in 10 mL aliquots
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