PES Stock Solution

NaNO3 50 mg
Na2-Glycerolphosphate•4H2O 50 mg
Tris Buffer 500 mg
Iron Solution 25 mL
Trace Metal 15 mL
Milli-Q Water 100 mL
  • Measure out Milli-Q
  • Add NaNO3 and Na2-Glycerolphosphate•4H2O
  • Combine trace metal and iron solutions in separate container
  • Autoclave both containers
  • Once cool, combine both mixtures
  • Store at 4oC in 10 mL aliquots