PES30 (Agar)

Instant Ocean 30 g
PES Stock 20 mL
Vitamin Solution 2 mL
(Agar 10 g)
Milli-Q Water ~1 L
  • Measure out Milli-Q
  • Add and dissolve Instant Ocean
  • Lower pH to approximately 3
  • Bubble overnight
  • Raise pH to 8.3
  • (Add agar)
  • Autoclave
  • Add PES stock and vitamin solution
  • Aliquot and store as needed

pH: 8.3