| Instant Ocean | 30 g |
| PES Stock | 20 mL |
| Vitamin Solution | 2 mL |
| (Agar | 10 g) |
| Milli-Q Water | ~1 L |
- Measure out Milli-Q
- Add and dissolve Instant Ocean
- Lower pH to approximately 3
- Bubble overnight
- Raise pH to 8.3
- (Add agar)
- Autoclave
- Add PES stock and vitamin solution
- Aliquot and store as needed
pH: 8.3
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