-Pi Stock

MgSO4•7H2O 20 g
CaCl2•2H2O 6 g
NaCl 20 g
Microelement Stock 500 mL
Milli-Q Water 1.5 L

 

  •  Dilute first three ingredients separately into 500 mL of Milli-Q
  • Autoclave three solutions, and allow to cool
  • Combine four solutions in order, allowing each to dissolve
  • Store at 4oC, shake well before use, do not autoclave