| Trizma Base | 4.844 g |
| EDTA | 372.2 mg |
| Glacial Acetic Acid | 1.15 mL |
| Milli-Q Water | ~1 L |
- Measure out approximately 300 mL Milli-Q
- Add all components in order
- Bring volume up to 1 L with Milli-Q
- Adjust pH to 8.3
- Store at 4oC
| Trizma Base | 4.844 g |
| EDTA | 372.2 mg |
| Glacial Acetic Acid | 1.15 mL |
| Milli-Q Water | ~1 L |