TBE

Trizma Base 12.11 g
Boric Acid, anhydrous 5.565 g
EDTA 372 mg
Milli-Q Water ~1 L

 

  • Measure out approximately 300 mL Milli-Q
  • Add all components in order
  • Bring volume up to 1 L with Milli-Q
  • Adjust pH to 8.3
  • Store at 4o