TE Buffer

1M Tris 5 mL
0.5M EDTA 1 mL
Milli-Q Water ~500 mL

 

  • Measure out 490 mL Milli-Q
  • Add components in order
  • Adjust pH to 7.4 using HCl
  • Bring volume to 500 mL
  • Autoclave for 30 minutes
  • Store at 4oC